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mouse anti pk sv5 pk1  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse anti pk sv5 pk1
    Mouse Anti Pk Sv5 Pk1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sv5+pk1/Mouse+anti+Simian+Virus+5/pmc12802923-64-14-18
    Average 86 stars, based on 12 article reviews
    mouse anti pk sv5 pk1 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Polyubiquitin architecture editing on collided ribosomes maintains persistent RQC activity
    Article Snippet: Proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore), blocked with 5% skim milk, and incubated with antibodies. .. The following primary and secondary antibodies were used: Anti-HA-Peroxidase from Roche (# 12013819001, RRID: AB 390917); Anti-FLAG M2 from Sigma-Aldrich (# F1804); Anti-eEF3 antibody (Lab stock); Mouse Anti-Viral V5-TAG monoclonal antibody, Unoconjugated, Clone SV5-Pk1 from Bio-Rad (# MCA1360, AB_322378); Anti-Ubiquitin (P4D1) HRP from Santa Cruz Biotechnology (# sc-0817, RRID:AB_628423); Anti-K48 Ubiquitin antibody from Cell Signaling Technology (# 8081; RRID:AB_1587580); Anti-K63 Ubiquitin antibody from EMD Millipore (# 05-1308; RRID:AB_10859893); ECL Anti-mouse (# NA931V) and Anti-rabbit (# NA934V) IgG, horseradish Peroxidase (HRP)-conjugated secondary antibodies from GE Healthcare. .. The membrane was then washed and reacted with the homemade ImmunoStar solution.

    Article Title: Editing of the polyubiquitin architecture on the collided ribosome maintains persistent RQC activity
    Article Snippet: Proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore), blocked with 5% skim milk and incubated with antibodies. .. The following primary and secondary antibodies were used: Anti-HA-Peroxidase from Roche (# 12013819001, RRID: AB 390917); Anti-FLAG M2 from Sigma-Aldrich (# F1804); Anti-eEF3 antibody (Lab stock); Mouse Anti-Viral V5-TAG monoclonal antibody, Unoconjugated, Clone SV5-Pk1 from Bio-Rad (# MCA1360, AB_322378); Anti-Ubiquitin (P4D1) HRP from Santa Cruz Biotechnology (# sc-0817, RRID:AB_628423); Anti-K48 Ubiquitin antibody from Cell Signaling Technology (# 8081; RRID:AB_1587580); Anti-K63 Ubiquitin antibody from EMD Millipore (# 05-1308; RRID:AB_10859893); ECL Anti-mouse (# NA931V) and Anti-rabbit (# NA934V) IgG, horseradish Peroxidase (HRP)-conjugated secondary antibodies from GE Healthcare. .. The membrane was then washed and reacted with homemade ImmunoStar solution.

    Article Title: Polyubiquitin architecture editing on collided ribosomes maintains persistent RQC activity.
    Article Snippet: Proteins were separated by SDSPAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore), blocked with 5% skim milk, and incubated with antibodies. .. The following primary and secondary antibodies were used: Anti-HAPeroxidase from Roche (# 12013819001, RRID: AB 390917); AntiFLAG M2 from Sigma-Aldrich (# F1804); Anti-eEF3 antibody (Lab stock); Mouse Anti-Viral V5-TAG monoclonal antibody, Unoconjugated, Clone SV5-Pk1 from Bio-Rad (# MCA1360, AB_322378); AntiUbiquitin (P4D1) HRP from Santa Cruz Biotechnology (# sc-0817, RRID:AB_628423); Anti-K48 Ubiquitin antibody from Cell Signaling Technology (# 8081; RRID:AB_1587580); Anti-K63 Ubiquitin antibody from EMD Millipore (# 05-1308; RRID:AB_10859893); ECL Anti-mouse (# NA931V) and Anti-rabbit (# NA934V) IgG, horseradish Peroxidase (HRP)-conjugated secondary antibodies from GE Healthcare. .. The membrane was then washed and reacted with the homemade ImmunoStar solution.

    Chromatin Immunoprecipitation:

    Article Title: Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
    Article Snippet: Variation in brain wiring contributes to non-heritable behavioral individuality.. How and when these individualized wiring patterns emerge and stabilize during development remains unexplored.. In this study, we investigated the axon targeting dynamics of Drosophila visual projecting neurons called DCNs/LC14s, using four-dimensional live-imaging, mathematical modeling and experimental validation.

    Flow Cytometry:

    Article Title: Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
    Article Snippet: Variation in brain wiring contributes to non-heritable behavioral individuality.. How and when these individualized wiring patterns emerge and stabilize during development remains unexplored.. In this study, we investigated the axon targeting dynamics of Drosophila visual projecting neurons called DCNs/LC14s, using four-dimensional live-imaging, mathematical modeling and experimental validation.

    Article Title: The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry
    Article Snippet: .. In short, cells were stained with anti-V5 tag antibody clone SV5-Pk1 (Bio-Rad) conjugated to AF488 (2.9 μg/mL) on ice for 1 h. After three washing steps with FACS buffer, cells were resuspended in 100 μL FACS buffer and analyzed on a LSR-Fortessa flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) using 405 and 488 nm excitation lasers and bandpass filters for BV421 (450/40 nm) and FITC (530/30 nm). .. The Quantum MESF kit Alexa Fluor 488 (Bangs Laboratories, Fishers, IN, USA) beads were analyzed in the flow cytometer on the same day in FACS buffer.

    Magnetic Resonance Imaging:

    Article Title: Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
    Article Snippet: Variation in brain wiring contributes to non-heritable behavioral individuality.. How and when these individualized wiring patterns emerge and stabilize during development remains unexplored.. In this study, we investigated the axon targeting dynamics of Drosophila visual projecting neurons called DCNs/LC14s, using four-dimensional live-imaging, mathematical modeling and experimental validation.

    Biomarker Discovery:

    Article Title: Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster.
    Article Snippet: Variation in brain wiring contributes to non-heritable behavioral individuality.. How and when these individualized wiring patterns emerge and stabilize during development remains unexplored.. In this study, we investigated the axon targeting dynamics of Drosophila visual projecting neurons called DCNs/LC14s, using four-dimensional live-imaging, mathematical modeling and experimental validation.

    Staining:

    Article Title: The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry
    Article Snippet: .. In short, cells were stained with anti-V5 tag antibody clone SV5-Pk1 (Bio-Rad) conjugated to AF488 (2.9 μg/mL) on ice for 1 h. After three washing steps with FACS buffer, cells were resuspended in 100 μL FACS buffer and analyzed on a LSR-Fortessa flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) using 405 and 488 nm excitation lasers and bandpass filters for BV421 (450/40 nm) and FITC (530/30 nm). .. The Quantum MESF kit Alexa Fluor 488 (Bangs Laboratories, Fishers, IN, USA) beads were analyzed in the flow cytometer on the same day in FACS buffer.

    FACS:

    Article Title: The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry
    Article Snippet: .. In short, cells were stained with anti-V5 tag antibody clone SV5-Pk1 (Bio-Rad) conjugated to AF488 (2.9 μg/mL) on ice for 1 h. After three washing steps with FACS buffer, cells were resuspended in 100 μL FACS buffer and analyzed on a LSR-Fortessa flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA) using 405 and 488 nm excitation lasers and bandpass filters for BV421 (450/40 nm) and FITC (530/30 nm). .. The Quantum MESF kit Alexa Fluor 488 (Bangs Laboratories, Fishers, IN, USA) beads were analyzed in the flow cytometer on the same day in FACS buffer.

    Article Title: PET-based tracking of CAR T cells and viral gene transfer using a cell surface reporter that binds to lanthanide complexes
    Article Snippet: MACS was done using a MiniMACS starting kit with MS columns and anti-mouse IgG MicroBeads (both Miltenyi Biotec). .. Approximately 1 × 10 7 cells were labelled with 1 μg ml −1 mouse anti-V5-tag antibody in 2 ml (clone SV5-Pk1, Bio-Rad) for 1 h at 4 °C and washed twice with FACS buffer. ..

    Article Title: PET-based tracking of CAR T cells and viral gene transfer using a cell surface reporter that binds to lanthanide complexes.
    Article Snippet: MACS was done using a MiniMACS starting kit with MS columns and anti-mouse IgG MicroBeads (both Miltenyi Biotec). .. Approximately 1 × 107 cells were labelled with 1 μg ml−1 mouse anti-V5-tag antibody in 2 ml (clone SV5-Pk1, Bio-Rad) for 1 h at 4 °C and washed twice with FACS buffer. ..



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